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哈靈文獻：熱烈祝賀重慶醫科大學陳強老師文獻發表成功

熱烈祝賀重慶醫科大學陳強老師文獻發表成功

文獻主題：

腭部間充質干細胞生物學行為的比較

2,3,7,8-四氯二苯并對二惡英體外誘導的上皮細胞

以下是相關指標索引

Twenty female C57BL/6 mice, 8–10 weeks of age, from Chongqing

Medical University Animal Center were used in this study. They were

housed under constant conditions at a temperature of 22 ± 2 °C, hu

midity of 50 ± 10 %, and 12 -h light/dark cycle. Female mice were

crossed with males in the evening and checked for vaginal plugs the

next morning. Additionally, their weights were recorded (embryonic

day 0, E0). On gestation day 14 (GD14), pregnant mice with weight

gain greater than 2 g were sacrifificed for primary mesenchymal cells. All

experiments abided by the Institutional Ethical Guidelines for

Experiments with Animals.

2.2. Cells and medium

Epithelial cells and medium were obtained from Shanghai Haling

Biotechnology Company, Limited. Primary epithelial cells were derived

from the palatal tissue of GD14 embryos from C57BL/6 mice.

Immunoflfluorescence was performed to detect the expression of the

epithelial marker ZO-1, and microscopic morphology was used to

confifirm its shape. A specifific medium was used to grow the epithelial

cells.

Mesenchymal cells were obtained from the palatal tissue of GD14

embryos from C57BL/6 mice. The methods of mesenchymal cell ac

quisition and culture were according to the previous study (Feng et al.,

2013). Immunoflfluorescence was performed to detect the expression of

the mesenchymal marker vimentin, and microscopic morphology was

used to confifirm its form. The media were DMEM/F12 (1:1) basic (1×)

(Gibco, USA) with 10 % fetal calf serum (FBS, Sijiqing, China).

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