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哈靈文獻:三角帆蚌的HcTyr和HcTyp-1
熱烈祝賀 上海海洋大學 -陳夏君 陳老師 文獻發表成功
文獻主題:
三角帆蚌的HcTyr和HcTyp-1
文獻中引用的我司產品(加亮部分):
Themantle samples (0.5 g of themantle center and 0.5 g of themantle
pallial and mantle edge from each sample) from eight healthy
H. cumingii specimens (four purple mussels and four white mussels)
were collected and immediately frozen in liquid nitrogen and stored
at−80 °C until use. The tyrosinase activity was estimated using the tissue
tyrosinase activity assay kit (HaLing, Shanghai, China) according to
在−80°C下使用。酪氨酸酶活性用組織學方法測定
酪氨酸酶活性測定試劑盒(上海哈靈生物科技有限公司)
themanufacturer's instructions. In brief, the sampleswere comminuted
in some liquid nitrogen with a mortar and pestle and treated with the
lysis buffer. The solution was centrifuged, and the supernatant was collected
andmixed with buffer and substrate (tyrosine). Finally, the samples
were incubated at 25 °C for 60 min, and tyrosinase activity was
measured using a spectrophotometer with the dopachrome content at
475 nm (UV-3010; Shimadzu, Japan). Tyrosinase catalyzes a reaction
that converts tyrosine to dopachrome by hydroxylation and then to
dopaquinone. One unit of tyrosinase activitywas defined as the amount
of activity that produced 1 μmol of dopachrome per minute via the hydroxylation
of tyrosine by tyrosinase at 25 °C. One-way ANOVAwas performed
using SPSS 17.0 to determine whether there were any
significant differences. Data from the TYR activity experiments were
expressed as the mean± SD (n=5) values. Significance was accepted
at the level of p b 0.05.
文獻鏈接:
鏈接: https://pan.baidu.com/s/1NqU0epVjnHvB942Nth1Wxg 提取碼: a24d